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    About 16S rRNA Bacterial flora analysis

    Service Overview

    Next-generation sequencers are used to sequence regions of high genetic diversity in bacterial 16S rRNA genes to identify and estimate the proportion of bacterial flora. Negative controls are used to identify background bacteria of environmental origin and a report containing the correlation between samples and a negative control, is included. In addition, contamination by environmentally-derived bacteria brought in from reagents is minimized, so even samples with low amounts of bacteria can be analyzed.

    Features

    ・Analysis from a single sample is possible at our laboratory in Japan.
    ・Analysis of low-biomass samples with small amounts of bacteria is possible using a special method developed by us.
    ・Bacteria are identified using the world standard Silva database and the germline databases.
    ・Analysis down to the species level is possible.
    ・Well-versed with various clinical specimens including vaginal, uterine tissue, breast milk, saliva, and oral cavity tissues.
    ・May be able to perform Test on non-fresh samples (FFPE sections, Gram stain slides, etc.)
    ・Statistical analysis for papers and conferences is also available (consultation required, additional cost)

    Flow of 16S rRNA bacterial analysis Service

    • 01

      Interview

      The contact person will ask you for your specimen information, analysis details, delivery date, etc., after which we will send you a project request form.

    • 02

      Ordering and Sample Shipping

      After you confirm the details of your analysis request, please place an order and ship the specimen to our laboratory.

    • 03

      DNA extraction

      If there is a problem with the quality of the DNA, we may contact you.

    • 04

      Sequence

      DNA sequencing is performed using a next-generation sequencer.

    • 05

      Data Analysis

      Bioinformatics tools will be used to identify and estimate the proportion of flora at the species level.

    • 06

      Delivery

      The analysis results will be delivered as a report (in principle, within 20 business days).
      The fastq and OTU representative sequences obtained as intermediate files of the analysis can also be delivered upon request.